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"registry+https://github.com/rust-lang/crates.io-index" +checksum = "589f6da84c646204747d1270a2a5661ea66ed1cced2631d546fdfb155959f9ec" diff --git a/rust/tagfinder/Cargo.toml b/rust/tagfinder/Cargo.toml new file mode 100644 index 0000000..97eff0f --- /dev/null +++ b/rust/tagfinder/Cargo.toml @@ -0,0 +1,7 @@ +[package] +name = "tagfinder" +version = "0.1.0" +edition = "2021" + +[dependencies] +clap = { version = "4.5.20", features = ["derive"] } diff --git a/rust/tagfinder/Makefile b/rust/tagfinder/Makefile new file mode 100644 index 0000000..34892ff --- /dev/null +++ b/rust/tagfinder/Makefile @@ -0,0 +1,2 @@ +run: + cargo run -- --families test/samples/families.tsv --positions test/samples/position.tsv --definitions 0,1 \ No newline at end of file diff --git a/rust/tagfinder/src/finder.rs b/rust/tagfinder/src/finder.rs new file mode 100644 index 0000000..f7fa48c --- /dev/null +++ b/rust/tagfinder/src/finder.rs @@ -0,0 +1,135 @@ +use std::cmp::Ordering; +use std::collections::HashMap; +use std::fs::File; +use std::io::{self, BufRead}; +use std::path::Path; + +fn read_lines

(filename: P) -> io::Result>> +where + P: AsRef, +{ + let file = File::open(filename)?; + Ok(io::BufReader::new(file).lines()) +} + +pub fn parse_families

(filename: P) -> HashMap +where + P: AsRef, +{ + let mut map: HashMap = HashMap::new(); + if let Ok(lines) = read_lines(filename) { + for line in lines.flatten() { + let parts: Vec<&str> = line.split("\t").collect(); + let gene = parts[0]; + let family: u64 = parts[1].parse().unwrap(); + map.insert(gene.to_string(), family); + } + } + map +} + +pub fn parse_position

(filename: P) -> Vec<(String, String, u64, u64)> +where + P: AsRef, +{ + let mut vec: Vec<(String, String, u64, u64)> = Vec::new(); + if let Ok(lines) = read_lines(filename) { + for line in lines.flatten() { + let parts: Vec<&str> = line.split("\t").collect(); + let gene = parts[0]; + let chromosome = parts[1]; + let start: u64 = parts[2].parse().unwrap(); + let end: u64 = parts[3].parse().unwrap(); + vec.push((gene.to_string(), chromosome.to_string(), start, end)); + } + } + vec +} + +pub fn compare_gene_position( + position_a: &(String, String, u64, u64), + position_b: &(String, String, u64, u64), +) -> Ordering { + let (_gene_a, chromosome_a, start_a, _end_b) = position_a; + let (_gene_b, chromosome_b, start_b, _end_b) = position_b; + if chromosome_a < chromosome_b { + return Ordering::Less; + } else if chromosome_a > chromosome_b { + return Ordering::Greater; + } else { + if start_a < start_b { + return Ordering::Less; + } else { + return Ordering::Greater; + } + } +} + +pub fn detect_tag_of_definitions( + definitions: &Vec, + gene_positions: &Vec<(String, String, u64, u64)>, + gene_families: &HashMap, +) -> HashMap>)> { + let mut tag_numbers: HashMap>)> = HashMap::new(); + for definition in definitions { + let tag_numbers_def = detect_tag_of_definition(*definition, gene_positions, gene_families); + for (gene, (family, tag)) in tag_numbers_def { + if tag_numbers.contains_key(&gene) { + let (_gene, tags) = tag_numbers.get_mut(&gene).unwrap(); + tags.push(tag); + } else { + tag_numbers.insert(gene, (family, vec![tag])); + } + } + } + tag_numbers +} + +pub fn detect_tag_of_definition( + definition: u64, + gene_positions: &Vec<(String, String, u64, u64)>, + gene_families: &HashMap, +) -> HashMap)> { + let mut tag_numbers: HashMap)> = HashMap::new(); + let mut spacer_index: u64 = 0; + let mut tag_index: u64 = 0; + for (i, (gene_i, chromosome_i, _start_i, _end_i)) in gene_positions.iter().enumerate() { + let family_repr: String; + let mut is_on_tag: bool = false; + if let Some(family_i) = gene_families.get(gene_i) { + family_repr = format!("{}", family_i); + let mut nb_intra_tag_spacers: u64 = 0; + if !tag_numbers.contains_key(gene_i) { + for (gene_j, chromosome_j, _start_j, _end_j) in &gene_positions[(i + 1)..] { + if *chromosome_i != *chromosome_j { + break; + } + if let Some(family_j) = gene_families.get(gene_j) { + if *family_i == *family_j { + nb_intra_tag_spacers = 0; + if !is_on_tag { + is_on_tag = true; + tag_index += 1; + tag_numbers.insert(gene_i.clone(), (family_repr.clone(), Some(tag_index))); + } + tag_numbers.insert(gene_j.clone(), (family_repr.clone(), Some(tag_index))); + } + } else { + nb_intra_tag_spacers += 1; + } + if nb_intra_tag_spacers > definition { + break; + } + } + if !is_on_tag { + tag_numbers.insert(gene_i.clone(), (family_repr, None)); + } + } + } else { + spacer_index += 1; + family_repr = format!("spacer{}", spacer_index); + tag_numbers.insert(gene_i.clone(), (family_repr, None)); + } + } + tag_numbers +} diff --git a/rust/tagfinder/src/main.rs b/rust/tagfinder/src/main.rs new file mode 100644 index 0000000..73a7068 --- /dev/null +++ b/rust/tagfinder/src/main.rs @@ -0,0 +1,57 @@ +use std::{collections::HashMap, path::PathBuf}; +use clap::Parser; +use finder::{detect_tag_of_definitions, parse_families, parse_position}; + +mod finder; + +#[derive(Parser)] +#[command(version="0.0.1", about="Find Tandemly Arrayed Genes", long_about="Find Tandemly Arrayed Genes in a genome based on gene position and Tandemly Arrayed Genes definition (i. e. number of spacers)")] +struct Cli { + #[arg(short='f', long="families", value_name="FAMILIES FILE")] + families: PathBuf, + + #[arg(short='p', long="positions", value_name="POSITIONS FILE")] + positions: PathBuf, + + #[arg(short='d', long="definitions", value_name="TAGs DEFINITIONS", help="Comma seperated integers for the number of spacers to consider")] + definitions: String +} + +fn main() { + let cli = Cli::parse(); + + let gene_families: HashMap = parse_families(cli.families); + + let mut gene_positions: Vec<(String, String, u64, u64)> = parse_position(cli.positions); + + gene_positions.sort_by(|a, b | crate::finder::compare_gene_position(a, b)); + + let definitions: Vec = cli.definitions.split(",").map(|x| x.parse().unwrap()).collect(); + + let tag_numbers = detect_tag_of_definitions(&definitions, &gene_positions, &gene_families); + + // Print header + print!("gene\tfamily\t"); + for (i, definition) in definitions.iter().enumerate() { + print!("tag{}", definition); + if (i + 1) < definitions.len() { + print!("\t"); + } + } + print!("\n"); + + for (gene, (family, tags)) in tag_numbers { + print!("{}\t{}\t", gene, family); + for (i, tag) in tags.iter().enumerate() { + if let Some(tag) = tag { + print!("{}", tag); + } else { + print!("-"); + } + if (i + 1) < tags.len() { + print!("\t"); + } + } + print!("\n"); + } +} diff --git a/rust/tagfinder/test/samples/families.tsv b/rust/tagfinder/test/samples/families.tsv new file mode 100644 index 0000000..8d12e15 --- /dev/null +++ b/rust/tagfinder/test/samples/families.tsv @@ -0,0 +1,4 @@ +A 1 +B 1 +C 2 +D 2 diff --git a/rust/tagfinder/test/samples/position.tsv b/rust/tagfinder/test/samples/position.tsv new file mode 100644 index 0000000..7f9e0c0 --- /dev/null +++ b/rust/tagfinder/test/samples/position.tsv @@ -0,0 +1,6 @@ +A 1 1 1 +B 1 2 1 +C 2 3 1 +U 2 4 1 +V 2 5 1 +D 2 10 1 \ No newline at end of file