function lcsq_matrix(seq1, seq2) local gap_penalty = 0 local match_score = 1 local n1 = string.len(seq1) local n2 = string.len(seq2) -- Create a n1 x n2 matrix local matrix = {} for i=0,n1 do matrix[i] = {} for j=0,n2 do matrix[i][j] = 0 end end -- Fill the rest of the matrix local match, delete, insert for i=1,n1 do for j=1,n2 do if string.sub(seq1, i, i) == string.sub(seq2, j, j) then match = matrix[i-1][j-1] + match_score else match = matrix[i-1][j-1] end gap1 = matrix[i-1][j] + gap_penalty gap2 = matrix[i][j-1] + gap_penalty matrix[i][j] = math.max(match, gap1, gap2) end end return matrix end local function has_value (tab, val) for index, value in ipairs(tab) do if value == val then return true end end return false end function repr_matrix(matrix) repr = "" for i=0,#matrix do for j=0,#matrix[i] do repr = repr .. matrix[i][j] .. " " end repr = repr .. "\n" end return repr end function draw_lcsq_matrix_graph(seq1, seq2, matrix) local tikz_code = "" function coordinate(i, j) return i .. "_" .. j end local steps = { {-1, -1}, {0, -1}, {-1, 0}, } local n1 = string.len(seq1) local n2 = string.len(seq2) local path = {} local i = n1 local j = n2 while i >= 0 and j >= 0 do path[#path+1] = coordinate(i, j) local max = matrix[i][j] local max_step = steps[1] for index, step in ipairs(steps) do local k = i + step[1] local l = j + step[2] if k >= 0 and l >= 0 and matrix[k][l] > max then max_step = step max = matrix[k][l] end end i = i + max_step[1] j = j + max_step[2] end -- Draw the matrix as tikz node with matrix value for i=0,n1 do for j=0,n2 do local options = "" if has_value(path, coordinate(i, j)) then options = "[fill=gray, draw, minimum size=1]" end tikz_code = tikz_code .. "\\node" .. options .. " (" .. coordinate(i, j) .. ") at (" .. i .. ", " .. -j .. ")" .. " {" .. matrix[i][j] .. "};" end end -- Add nucleotide labels for i=1,n1 do local nt = string.sub(seq1, i, i) tikz_code = tikz_code .. "\\node at (".. i .. "," .. 1 .. ")" .. "{$" .. nt .."$};" end for i=1,n2 do local nt = string.sub(seq2, i, i) tikz_code = tikz_code .. "\\node at (" .. -1 .. ", " .. -i .. ")" .. "{$ ".. nt .."$};" end -- For seq2 for i=0,n1 do for j=0,n2 do local max = 0 for index, step in ipairs(steps) do local k = i + step[1] local l = j + step[2] if k >= 0 and l >= 0 and matrix[k][l] > max then max = matrix[k][l] end end local highlighted = false for index, step in ipairs(steps) do local k = i + step[1] local l = j + step[2] if k >= 0 and l >= 0 and matrix[k][l] == max then tikz_code = tikz_code .. "\\draw[->] (" .. coordinate(i, j) .. ")" .. " -- " .. "(" .. coordinate (k, l) .. ");" end end end end return tikz_code end function draw_lcsq_matrix(seq1, seq2) -- print(string.format(" Path: %s -> %s", seq1, seq2)) local matrix = lcsq_matrix(seq1, seq2) local n1 = string.len(seq1) local n2 = string.len(seq2) local repr = "" -- Draw the matrix as tikz nodes for i=0,n1-1 do for j=0,n2-1 do repr = repr .. " " .. string.format("\\node[draw, minimum width=1cm, minimum height=1cm] at (%d, -%d) {};", i, j, matrix[i][j]) end end -- Draw the sequence labels for i=1,n1 do repr = repr .. " " .. string.format("\\node at (%d, -%d) {%s};", i-1, -1, string.sub(seq1, i, i)) end for i=1,n2 do repr = repr .. " " .. string.format("\\node at (%d, -%d) {%s};", -1, i-1, string.sub(seq2, i, i)) end -- Add a path from the bottom right corner to the top left corner, following the minimum of the three possible moves at each step local i, j, value, previous_value i = n1-1 j = n2-1 repr = repr .. " " string.format("\\draw[-,line width=2, gray] (%d, -%d) --", i, j) while i > 0 and j > 0 do value = math.min(matrix[i-1][j-1], matrix[i-1][j], matrix[i][j-1]) if value == matrix[i-1][j-1] then i = i - 1 j = j - 1 elseif value == matrix[i-1][j] then i = i - 1 else j = j - 1 end repr = repr .. " " .. string.format(" (%d, -%d) -- ", i, j) end repr = repr .. " " .. string.format("(0, 0) -- (-1, 1);", i, j) return repr end function main() local seq1 = "ATCTGAT" local seq2 = "TGCATA" local matrix = lcsq_matrix(seq1, seq2) print(draw_lcsq_matrix_graph(seq1, seq2, matrix)) end -- main() return { lcsq_matrix=lcsq_matrix, draw_lcsq_matrix_graph=draw_lcsq_matrix_graph, draw_lcsq_matrix=draw_lcsq_matrix }